Scientist II, Protein Biology

Job description

Work Schedule Standard (Mon-Fri) Environmental Conditions Adherence to all Good Manufacturing Practices (GMP) Safety Standards, Cold Room/Freezers -22degreesF/-6degrees C, Laboratory Setting, Some degree of PPE (Personal Protective Equipment) required (safety glasses, gowning, gloves, lab coat, ear plugs etc.), Will work with hazardous/toxic materials Job Description Scientist II – Protein Biology About the Company Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, with annual revenue of approximately $44 billion. Our Mission is to enable our customers to make the world healthier, cleaner, and safer. Whether our customers are accelerating life sciences research, solving analytical challenges, growing efficiency in their laboratories, improving patient health through diagnostics, or developing and manufacturing life-changing therapies, we are here to support them. Our global team delivers an outstanding combination of innovative technologies, purchasing convenience, and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services, Patheon, and PPD. About the Team The Antibody Team is involved in engineering and development of antibodies, with a deep focus on reagent quality and specificity. The core activities of team include design, development, purification, conjugation and testing of antibodies in a variety of immunoassay applications using groundbreaking tools and technologies. The goal of the program is recombinant antibody development and creation of product specific information to promote research use. Key Responsibilities • Perform hands-on recombinant antibody development and validation in a dynamic, fast-paced R&D environment, ensuring high throughput and quality outputs. • Plan and execute experiments meticulously, maintaining accurate and timely records in data sheets and laboratory notebooks in compliance with best practices. • Actively mine the scientific literature and remain current with emerging trends in antibody engineering, molecular biology, and associated fields. • Compile and present data in both written and oral formats; actively contribute to team meetings and cross-functional discussions. • Adhere to experimental schedules and quality standards with strong attention to detail and a commitment to quality. • Develop next-generation methodologies and process improvements to advance and streamline recombinant antibody development and validation platforms. • Drive protocol conceptualization and development within the team, enabling successful transfer of workflows to operational teams. • Provide informal leadership, technical supervision, and mentoring to junior scientists—supporting experimental design, data interpretation, and presentation to collaborators and stakeholders. • Provide strategic inputs and mentorship to foster innovation and cross-team collaboration across the antibody development program. • Partner with supporting functions—including product management, program management, operations, and business development—to achieve program milestones. Qualifications & Experience Education • Applicants should have a PhD in molecular biology, cell biology, genetics, immunology, biotechnology, computational biology or biochemistry with 2 to 3 years of experience. Technical Skills & Experience • Immunoassay Applications: Demonstrated experience in immunoassay development and application testing, including ELISA, Western Blotting, Flow Cytometry, Immunocytochemistry (ICC), Co-immunoprecipitation (co-IP), and Immunohistochemistry (IHC). Advanced expertise in microscopy, spatial biology platforms, and multiplexed IHC/IF, with experience developing and analyzing high-content tissue imaging assays for protein expression and cellular phenotyping is a must. • Molecular Biology: Solid foundation in molecular biology techniques including nucleic acid extraction, PCR, cloning, sequence analysis, and vector design. Experience with next-generation cloning strategies, expression vector construction, codon optimization, and PCR primer design is highly valued. • Bioinformatics, Programming & AI literacy: Proficiency in bioinformatics tools such as BLAST, pairwise and multiple sequence analysis. Working knowledge of structural biology and protein modeling and visualization tools such as PyMol, Schrödinger, Discovery Studio or other such programs is a plus. Proficiency in deep learning models and/or knowledge of large language models (LLMs), prompt engineering, and experience in implementing these tools to biological questions is highly desirable • Protein Science & Antibody Engineering: Practical knowledge of protein purification, antibody engineering, rational design of mutants, and structure-function studies. • Cell Culture Techniques: Competency in standard mammalian or other specialized cell culture techniques supporting protein expression, antibody production, or related workflows. • Antibody Development Platforms: Theoretical or practical exposure to antibody development platforms such as hybridoma, single B-cell, display technologies, or other recombinant approaches. Experience leading or contributing to core facilities for molecular biology or sequencing is a plus. • Automation & High-Throughput Methods: Experience with automated liquid handling platforms, high-content imaging or other high-throughput methodologies is advantageous. • <